I. Theoretical background

Introduction to transmission electron microscopy, general construction of transmission electron microscopes; filament (Wehnelt), apertures, lenses, Cs, Cc and focal length, high tension; alignments, TEM imaging bright/dark field, resolution, in-focus, over and under focus, astigmatism (condenser, objective and diffraction astigmatism), contrast; digital cameras; filter technology; the highest resolution in Life Sciences, best practice in biology; sample preparation and cryo-electron microscopy. Future prospects of TEM in biomedicine.

II. Practical sessions

Apertures, condenser and objective apertures, SA-aperture; adjusting the filament, demonstration of bias, C1 and C2 lenses, lifetime of filament, consequences of high bias and current; specimen eucentric position, focusing the microscope, wobbler usage; optimizing brightness (light), high tension; correcting astigmatism, the methods used on minimum contrast used by material scientists and the Hole method in combination with under-focus/over-focus; direct alignments, alignment procedures, test specimens; dark field (conical), wobbler adjustment.