Program
Wednesday, 12 June 2024
Confocal in SUPER-RESOLUTION
| 08:45 – 09:00 | Introductory word, practical information & course organization |
| 09:00 – 09:40 | Ivan Novotný (IMG) | Resolution in light microscopy; high-res. confocal microscope |
| 09:40 – 10:00 | Coffee break |
| 10:00 – 10:40 | Ivan Novotný (IMG) | Uncovered deconvolution magics |
| 10:40 – 11:20 | Michaela Blažíková (IMG) | SR in silico: basics of computational approaches |
| 11:20 – 12:30 | Lunch break |
| 12:30 – 13:00 | Leica / Specion | Lightning: an adaptive deconvolution for SR images |
| 13:00 – 13:30 | Andor / OptiXs | SRRF Stream: spinning disc implementation |
| 13:30 – 14:00 | EVIDENT / SVEN Biolabs | SORA: SR solution for spinning disk |
| Afternoon practical sessions | |
| 14:00 – 15:50 | Practical sessions (3 parallel groups): Lightning (group1) | SORA (group2) | SRRF (group3) |
| 15:50 – 16:00 | Coffee break |
| 16:00 – 17:50 | Practical sessions (3 parallel groups): Lightning (group3) | SORA (group1) | SRRF (group2) |
| 17:50 – 18:00 | Coffee break |
| 18:00 – 19:50 | Practical sessions (3 parallel groups): Lightning (group2) | SORA (group3) | SRRF (group1) |
| 20:00 | WrapUp, discussion and sandwich dinner |
Thursday, 13 June 2024
Structured Illumination Power
| 09:00 – 09:40 | Martin Čapek (IMG) | Srtuctured illumination microscopy – SIM |
| 09:40 – 10:10 | Klaus Weisshart (ZEISS) | Lattice SIM: new robust approach |
| 10:10 – 10:30 | Coffee break |
| 10:30 – 11:00 | NIKON | Crest Optics: SIM solution for spinning disk |
| 11:00 – 11:45 | Rainer Heintzmann| Nonlinear SIM and image reconstruction (online) |
| 11:45 – 13:00 | Lunch break |
| Afternoon practical sessions | |
| 13:00 – 14:50 | Practical sessions (3 parallel groups): SIM (group1) | Lattice SIM (group2) | Crest Optics SIM (group3) |
| 14:50 – 15:00 | Coffee break |
| 15:00 – 16:50 | Practical sessions (3 parallel groups): SIM (group3) | Lattice SIM (group1) | Crest Optics SIM (group2) |
| 16:50 – 17:00 | Coffee break |
| 17:00 – 18:50 | Practical sessions (3 parallel groups): SIM (group2) | Lattice SIM (group3) | Crest Optics SIM (group1) |
| 19:00 – 19:30 | Transfer to the restaurant |
| 19:30 | Social event |
Friday, 14 June 2024
Nanoscopy
| 09:00 – 09:30 | Ivan Novotný (IMG) | Stimulated emission depletion – STED microscopy |
| 09:30 – 10:00 | Michaela Blažíková (IMG) | Single molecule localization microscopy – SMLM |
| 10:00 – 10:30 | Coffee break |
| 10:30 – 11:15 | Paolo Bianchini|Nanoscopy in biophysics (online) |
| 11:15 – 11:30 | ADVI mounting media for SR microscopy |
| 11:30 – 12:30 | Lunch break |
| Afternoon practical sessions | |
| 12:30 – 14:20 | Practical sessions (3 parallel groups): STED (group1) | dSTORM/3D PALM (group2) | Deconvolution (group3) |
| 14:20 – 14:30 | Coffee break |
| 14:30 – 16:20 | Practical sessions (2 parallel groups): STED (group3) | dSTORM/3D PALM (group1) | Deconvolution (group2) |
| 16:20 – 16:30 | Coffee break |
| 16:30 – 18:20 | Practical sessions (2 parallel groups): STED (group2) | dSTORM/3D PALM (group3) | Deconvolution (group1) |
| 18:30 | Final conclusion, closing remarks |