About the Course

The course will cover the basics in image data acquisition, processing and analysis including methods of stereology. Apart from a theoretical background, the emphasis will be put on practical experience. The participants will learn how to use free software package Fiji for basic or more advanced analyses, how to evaluate co-localizations, analyse data from FRAP and electron microscopy data, how to track particles in images or segment objects, methods of artificial intelligence for image segmentation will be presented as well. They will learn how to improve data by deconvolution in Huygens software. We will have a lesson with Imaris software together with practical exercises. Stand-alone practical tasks using Fiji and Huygens will be an important part of the course as well.

The course follows the Microscopy Methods in Biomedicine, the previous attendance of this course, however, is not necessary.

Language

The course will be taught in English.

Place of the Event

Institute of Molecular Genetics of the Czech Academy of Sciences, Vídeňská 1083, Prague 4 – Krč

Scientific Supervisors

  • Prof. RNDr. Pavel Hozák, DrSc. (Institute of Molecular Genetics, Prague)
  • RNDr. Jiří Janáček, Ph.D. (Institute of Physiology, Prague)

Coordinators

  • RNDr. Michaela Blažíková, Ph.D. (Institute of Molecular Genetics, Prague)
  • Ing. Martin Čapek, Ph.D. (Institute of Molecular Genetics, Prague)

Faculty

  • RNDr. Michaela Blažíková, Ph.D. (Institute of Molecular Genetics, Prague)
  • Ing. Martin Čapek, Ph.D. (Institute of Molecular Genetics, Prague)
  • Mgr. Vlada Filimoněnko,Ph.D. (Institute of Molecular Genetics, Prague)
  • RNDr. Barbora Radochová, Ph.D. (Institute of Physiology, Prague)
  • Prof. RNDr. Pavel Hozák, DrSc. (Institute of Molecular Genetics, Prague)
  • RNDr. Jiří Janáček, Ph.D. (Institute of Physiology, Prague)
  • Dr. Daniel Reisen (Bitplane)
  • Ing. Ivan Novotný, Ph.D. (Institute of Molecular Genetics, Prague)
  • Mgr. Jan Valečka (Institute of Molecular Genetics, Prague)