About the Practical Course
A practical, selective, 3-day course for 12-15 participants, covering a wide range of methods in light microscopy of living samples.
The course introduces highly useful imaging techniques for live cells and larger specimens. Its main aim is to compare different methods, both theoretically and practically, across a variety of imaging systems. Topics range from widefield and confocal imaging to super-resolution microscopy and intracellular dynamics.
Each day combines theoretical instruction with practical demonstrations of various approaches and instruments. The course is strongly practice-oriented, with more than five hours of hands-on sessions every day.
The course is intended for users with previous experience in light microscopy and focuses on practical applications through numerous interactive demonstrations. Plenary lectures provide the theoretical background for all techniques presented.
Highlight of taught methods | systems
- LIVE CELL SUPERRESOLUTION | Leica STELLARIS 8 TauSTED Xtend / Zeiss LSM 980 with Airyscan 2 / Zeiss Elyra7 with Lattice SIM
- FAST 3D IMAGING | Zeiss LSM Lightfield 4D / Zeiss Lattice Lightsheet 7
- PHOTO-KINETIC MEASUREMENTS | Leica STELLARIS 8 / Zeiss LSM 980 with Airyscan 2
- TIME RESOLVED MICROSCOPY | Leica STELLARIS 8 FLIM
- CONFOCAL 2-PHOTON | Leica TCS SP8
Looking forward to seeing you in Prague!
Organizing Team
Jan Valečka, Light Microscopy Core Facility, IMG
Helena Novotná, Light Microscopy Core Facility, IMG
