About the Course

The course will address fundamental aspects of image data acquisition, processing, and analysis, encompassing techniques in stereology. Alongside theoretical principles, the course will prioritize hands-on practical learning. Participants will gain proficiency in utilizing the freely available software package Fiji for both basic and advanced analyses. They will learn to segment objects in images, explore methods of employing artificial intelligence for image segmentation assess co-localizations, track particles, analyze data from kinetic experiments and write their own macros.

Additionally, participants will master techniques to enhance data quality through deconvolution using Huygens software. An interactive session featuring Imaris software will be complemented by practical exercises. Furthermore, this year introduces theoretical and practical session dedicated to interpretation and analysis of electron microscopy data, introducing the freely available MIB software. Independent practical tasks involving Fiji and Huygens will also constitute a significant component of the course.

While the course aligns with the Microscopy Methods in Biomedicine, prior attendance is not a prerequisite for participation.

Language

The course will be taught in English.

Place of the Event

Institute of Molecular Genetics of the Czech Academy of Sciences, Vídeňská 1083, Prague 4 – Krč

Scientific Supervisors

  • Prof. RNDr. Pavel Hozák, DrSc. (Institute of Molecular Genetics, Prague)
  • RNDr. Jiří Janáček, Ph.D. (Institute of Physiology, Prague)

Coordinators

  • RNDr. Michaela Blažíková, Ph.D. (Institute of Molecular Genetics, Prague)
  • Ing. Martin Čapek, Ph.D. (Institute of Molecular Genetics, Prague)

Faculty

  • RNDr. Michaela Blažíková, Ph.D. (Institute of Molecular Genetics, Prague)
  • Ing. Martin Čapek, Ph.D. (Institute of Molecular Genetics, Prague)
  • Mgr. Markéta Dalecká (Institute of Molecular Genetics, Prague)
  • Prof. RNDr. Pavel Hozák, DrSc. (Institute of Molecular Genetics, Prague)
  • RNDr. Jiří Janáček, Ph.D. (Institute of Physiology, Prague)
  • Ing. Ivan Novotný, Ph.D. (Institute of Molecular Genetics, Prague)
  • Mgr. Dominik Pinkas, Ph.D. (Institute of Molecular Genetics, Prague)
  • RNDr. Barbora Radochová, Ph.D. (Institute of Physiology, Prague)
  • Julia Schweizer (Oxford Instruments GmbH)
  • Mgr. Jan Valečka (Institute of Molecular Genetics, Prague)