About the Course
The course is designed for users of electron microscopes in biomedical field, both from scientific laboratories and from core facilities. The aim of the course is to provide an orientation to basic and special techniques in biomedical electron microscopy in an application-oriented manner. In the theroretical and practical sessions, sample preparation under room temperature and cryo conditions will be covered, including special workflows such as cryo-sectioning, freeze-fracture replica, or cryo-CLEM workflow on FIB-milled lamella. Besides basic imaging, special techniques such as (cryo)FIB-SEM, STEM tomography and EDS analysis will be demonstrated, with introduction to data processing and interpretation. Special emphasis will be placed on discussing how to choose the appropriate methodology for a specific scientific project.
Language
The course will be taught in English.
Place of the Event
Institute of Molecular Genetics of the Czech Academy of Sciences, Vídeňská 1083, Prague 4 – Krč
Course Coordinator
Dr. Vlada Filimoněnko (Institute of Molecular Genetics, Prague)
Faculty
- Dr. Vlada Filimoněnko (Institute of Molecular Genetics, Prague)
- Ing. Andreas Nowak (Leica Microsystems)
- Dr. Frédéric Leroux (Leica Microsystems)
- Dr. Saskia Mimietz-Oeckler (Leica Microsystems)
- Ing. Martin Uher (TESCAN Brno)
- Dr. Dominik Pinkas (Institute of Molecular Genetics, Prague)
- Mgr. Markéta Dalecká (Institute of Molecular Genetics, Prague)
- Dr. Oldřich Benada (Institute of Microbiology, Prague)
- Dr. Régis Ravelle-Chapuis (JEOL)
Looking forward to seeing you in Prague!
Organizing Team
