Program

Monday 18 May 2026

Meeting room 0.117
14:00 – 16:00Installations (voluntary if any problem arises)

Tuesday 19 May 2026

Lecture room 0.195
09:00 – 09:30Registration + Fast Installation Checking + Coffee
09:30 – 09:35Welcome
09:35 – 09:50Course overview – methods vs. applications | Martin Čapek (IMG)
09:50 – 10:10Anaconda + Python overview | Jan Valečka (IMG)
10:10 – 10:55Introduction into deep learning (DL) for image segmentation | Martin Čapek (IMG)
Coffee break
11:10 – 11:40Image denoising (Noise2Void)  | Jan Valečka (IMG)
11:40 – 12:20Short introduction into Python | Martin Čapek (IMG)
Lunch
13:20 – 14:50StarDist segmentation block | Martin Čapek (IMG)
– Fiji practicals using 2D/3D images of various images and quality.
– Python practicals (Jupyter notebook) using a large image.
14:50 – 16:05Cellpose segmentation block | Jan Valečka (IMG)
– Fiji practicals using 2D/3D images, macro example.
– Cellpose standalone app practicals, retraining the model for new images.
Coffee break
16:20 – 17:05arivis.cloud: free available cloud platform for image processing including machine and deep learning. Lecture and demo | Pavel Krist (Zeiss)
Sponsor’s lecture.
17:05End of the Day

Wednesday 20 May 2026

Lecture room 0.195
09:00 – 09:45Omnipose bacteria segmentation block | Michaela Blažíková (IMG)
– Omnipose standalone app practicals using bacteria time-lapse data.
– Python practicals (Jupyter notebook).
09:45 – 10:45TrackMate block: tracking using DL segmentation models | Michaela Blažíková (IMG)
– Short intro.
– Fiji practicals using time-lapse images.
Coffee break
11:00 – 13:00MitoSegNet block: mitochondrial segmentation and morphology analysis | Martin Čapek (IMG)
Demo of the full process of DL-based analysis including image annotations, model retraining and morphological analysis.

– MitoSegNet standalone app – mitochondrial segmentation.
– Labkit plugin in Fiji – annotations of new mitochondrial images.
– MitoSegNet standalone app – retraining the model by using the annotated images.
– MitoSegNet standalone app – statistical morphological analysis of new segmented images.
Lunch
14:00 – 14:30DeepImageJ – various applications of DL methods to image processing | Michaela Blažíková (IMG)
14:30 – 16:00Google Colab block: cloud-based distant computing | Michaela Blažíková (IMG)
– Delta2 – bacteria tracking package using DL segmentation/tracking models.
– ZeroCostDL4Mic practicals demos to process microscopy data.
Coffee break
16:15 – 16:45Virtual Reality based image segmentation and measurement: a Toy Demo | Pavel Krist (Zeiss)
Sponsor’s practicals.
16:45End of the Day

Thursday 21 May 2026

Lecture room 0.195
09:00 – 11:00Independent solving of practical tasks based on the lessons taught + discussions, Part 1
Coffee break
11:15 – 13:15Independent solving of practical tasks based on the lessons taught + discussions, Part 2
13:15 – 13:30Course evaluation
13:30End of the Course